Revised 06/09/20178 9
• High-contrast gels and blots are discouraged, as
overexposure may mask additional bands. Authors should
strive for exposures with gray backgrounds. Immunoblots
should be surrounded by a black line to indicate the borders
of the blot, if the background is faint.
• For quantitative comparisons, appropriate reagents, controls
and imaging methods with linear signal ranges should be
used.
Microscopy adjustments should be applied to the entire image.
Threshold manipulation, expansion or contraction of signal ranges
and the altering of high signals should be avoided. If ‘pseudo-
colouring’ and nonlinear adjustment (for example ‘gamma
changes’) are used, this must be disclosed. Adjustments of
individual colour channels are sometimes necessary on ‘merged’
images, but this should be noted in the figure legend. We
encourage inclusion of the following with the final revised version
of the manuscript for publication:
• In the Methods section, specify the type of equipment
(microscopes/objective lenses, cameras, detectors, filter
model and batch number) and acquisition software used.
Although we appreciate that there is some variation between
instruments, equipment settings for critical measurements
should also be listed.
• The display lookup table (LUT) and the quantitative map
between the LUT and the bitmap should be provided,
especially when rainbow pseudo-colour is used. It should be
stated if the LUT is linear and covers the full range of the
data.
• Processing software should be named and manipulations
indicated (such as type of deconvolution, three-dimensional
reconstructions, surface and volume rendering, 'gamma
changes', filtering, thresholding and projection).
• Authors should state the measured resolution at which an
image was acquired and any downstream processing or
averaging that enhances the resolution of the image.
Availability of Data and Materials
An inherent principle of publication is that others should be able to
replicate and build upon the authors’ published claims. Therefore,
a condition of publication is that authors are required to make
materials, data, and associated protocols available in a publicly
accessible database. Where one does not exist, the information must
be made available to referees at submission and to readers
promptly upon request. Any restrictions on material availability or
other relevant information must be disclosed in the manuscript’s
Methods section and should include details of how materials and
information may be obtained.
Sequences, Structures and “Omics”
Papers reporting protein or DNA sequences and molecular
structures will not be accepted without an accession number
to Genbank/EMBL/DDBJ, SWISS-PROT
,
ProteinDataBank, or other publicly available database in general
use in the field that gives free access to researchers from the date of
publication.
Authors of papers describing structures of biological
macromolecules must provide experimental data upon the request
of Editor if they are not already freely accessible in a publicly
available database such as ProteinDataBank
,
Biological Magnetic Resonance Databank, or Nucleic Acid
Database.
Human and Other Animal Experiments
For primary research manuscripts reporting experiments on live
vertebrates and/or higher invertebrates, the corresponding author
must confirm that all experiments were performed in accordance
with relevant guidelines and regulations.
All manuscripts reporting animal research must be written up in
accordance with the ARRIVE (Animal Research: Reporting In Vivo
Experiments) guidelines – see http://www.nc3rs.org/ARRIVE.
The manuscript must include in the Supplementary Information
(methods) section (or, if brief, within of the print/online article at an
appropriate place), a statement identifying the institutional and/or
licensing committee approving the experiments, including any
relevant details regarding animal welfare, patient anonymity, drug
side effects and informed consent.
For experiments involving human subjects, authors must identify
the committee approving the experiments, and include with their
submission a statement confirming that informed consent was
obtained from all subjects.
Biosecurity Policy
The Editor may seek advice about submitted papers not only from
technical reviewers but also on any aspect of a paper that raises
concerns. These may include, for example, ethical issues or issues of
data or materials access. Occasionally, concerns may also relate to
the implications to society of publishing a paper, including threats
to security. In such circumstances, advice will usually be sought
simultaneously with the technical peer-review process. As in all
publishing decisions, the ultimate decision whether to publish is the
responsibility of the editor of the journal concerned.
Peer Review
Manuscripts sent out for peer review are evaluated by at least one
independent reviewer (often two or more). Authors are welcome to
suggest independent reviewers to evaluate their manuscript, as well
as request individuals or laboratories. All recommendations are
considered, but it is at the Editor’s discretion their choice of
reviewers. To expedite the review process, only papers that seem
most likely to meet editorial criteria are sent for external review.
Papers judged by the editors to be of insufficient general interest or
otherwise inappropriate are rejected promptly without external
review. The editors then make a decision based on the reviewers'
evaluations:
• Accept, with or without editorial revisions.
• Revise, with the author addressing concerns raised by the
reviewers before a final decision is reached.
• Reject, but indicate to the authors that further work might
justify a resubmission.
• Reject outright, typically on grounds of specialist interest,
lack of novelty, insufficient conceptual advance or major
technical and/or interpretational problems.
Anonymity and Confidentiality
All details about submitted manuscripts are kept confidential and
no comments are issued to outside parties or organizations about
manuscripts under consideration or if they are rejected. Editors are
restricted to making public comments on a published article’s
content and their evaluation.