Prostate tissue section stained with Hematoxylin & Eosin Stain Kit.

Procedure

1. Deparaffinize sections, if required, and hydrate to distilled water.

2. Apply adequate Hematoxylin to completely cover tissue section and

incubate for 5 minutes.

3. Rinse slide in 2 changes of distilled water (15 seconds each) to

remove excess stain.

4. Apply adequate Bluing Reagent to completely cover tissue section

and incubate for 10–15 seconds.

10. Clear and coverslip.

Notes

a) Intensity should be optimized for each tissue type, fixative used,

and presentation (frozen or paraffin-embedded)

b) Use standard laboratory precautions to avoid contact with skin

and eyes.

c) Harmful if swallowed. Do not take internally.

d) These reagents are for research use only.

e) Follow all Federal, State and Local regulations for disposal.

Related Reagents

For research use only. Not intended for animal or human therapeutic or diagnostic use.

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H-3502.LBL-02405.Rev.00

Hematoxylin and Eosin Stain Kit

USER GUIDE

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Cat. No.

Storage

H-3502

15–25 °C

The Hematoxylin and Eosin Stain Kit is intended for

use in histology and cytology applications. Included

in this kit is a modified Eosin that provides the

benefits of a traditional alcoholic formulation with

significant improvements in usability. Advantages

include lower evaporation rate, better color patterns,

and improved surface tension to remain on tissue

section. Our Hematoxylin produces crisp, intense

blue nuclei providing optimal contrast to the Eosin

stained cytoplasm.

Colon, Prostate