Purification Beads, Columns & Resins Brochure

Purification Beads, Columns & Resins

SMALL-SCALE, HIGH-THROUGHPUT APPLICATIONS AND

LARGE SCALE PURIFICATION STRATEGIES

www.neb.uk.com

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Introduction

Isolation of pure substrates or proteins for downstream experiments is a common, yet time

consuming, task. New England Biolabs offers a variety of resins and magnetic beads that

are easy-to-use, highly specific, and available in several different formats for rapid isolation

and purification of proteins, nucleic acids and immunoglobulins. NEB’s magnetic beads are

ideally suited for applications involving high-throughput proteomic screening, small-scale

protein isolation, immunomagnetic isolations or cell separation experiments. With magnetic

beads, affinity purification of tagged proteins, antigens, antibodies and nucleic acids can be

done conveniently and quickly. Immobilized substrates remain biologically active and can

be eluted in small volumes or serve as ligands in subsequent pull-down or target interaction

experiments involving DNA or proteins. NEB’s resins enable simple, one-step purification

strategies for tagged proteins, and result in a high yield of highly pure substrate. For the

full list of products available for protein expression and purification, visit www.neb.com/

ProteinExpression.

PROTEIN

PURIFICATION

LARGE-SCALE

PURIFICATIONS

USE IN AUTOMATED

CHROMATOGRAPHY

HIGH-

THROUGHPUT

BIOTINYLATED

SUBSTRATE BINDING

PROTEIN

PULL-DOWN

NUCLEIC ACID

PULL-DOWN

mRNA PURIFICATION/

PULL-DOWN IMMUNOPRECIPITATION

CELL SEPARATION/

CELL SORTING

NEBExpress

™

Ni-NTA

Magnetic Beads

(NEB #S1423)

(His-tag)

l l

NEBExpress

Ni Spin Columns

(NEB #S1427)

(His-tag)

l l

NEBExpress Ni Resin

(NEB #S1428)

(His-tag)

l l l

Amylose Resin

(NEB #E8021)

(MBP)

l l

Amylose Resin High

Flow (NEB #E8022)

(MBP)

l l l

Amylose Magnetic

Beads (NEB #E8035)

(MBP)

l l

Anti-MBP Magnetic

Beads (NEB #E8037)

(MBP)

l l

Chitin Resin

(NEB #S6651)

(intein-CBD tag)

l l

Chitin Magnetic Beads

(NEB #E8036)

(intein-CBD tag)

l l

Oligo d(T)

Magnetic

Beads (NEB #S1419)

l l l

Streptavidin Magnetic

Beads (NEB #S1420)

l l

(biotinylated bait)

Hydrophilic

Streptavidin Magnetic

Beads (NEB #S1421)

l l

(biotinylated bait)

Protein A Magnetic

Beads (NEB #S1425)

l l

Protein G Magnetic

Beads (NEB #S1430)

l l

Goat Anti-Mouse IgG

Magnetic Beads

(NEB #S1431)

(Mouse lgGs)

Goat Anti-Rabbit IgG

Magnetic Beads

(NEB #S1432)

(Rabbit lgGs)

Goat Anti-Rat IgG

Magnetic Beads

(NEB #S1433)

(Rat lgGs)

Magnetic mRNA

Isolation Kit

(NEB #S1550)

l l

Product Selection Chart

table of contents

Polyhistidine-tagged Protein

Purification

Maltose Binding Protein (MBP)

Purification

Chitin Binding Domain (CBD)

Purification

SNAP-tag Purification

Magnetic Beads

Magnetic Separation Racks

3–4

5–6

8–11

INTRODUCTION

features

• Suitable for high-throughput and scalable

purificationstrategies

• High specific binding yields purities of > 95% in a single-

purification step

• Nitrilotriacetic acid (NTA) coordination exhibits low nickel

ion leaching

• Tolerates a wide range of conditions, including the presence

of protein denaturants and detergents. Compatible with

commercially available detergent-based cell lysis reagents

• Elution can be achieved by protonation, ligand exchange

(with imidazole) or extraction of the metal ion by a strong

chelator (e.g., EDTA)

• Includes all required buffers for purification

NEBExpress

™

Ni-NTA Magnetic Beads

NEBExpress Ni-NTA magnetic beads are an affinity matrix for the small-scale isolation

and purification of polyhistidine-tagged (His-tagged) fusion proteins in manual or auto-

mated formats. They are prepared with agarose-based, super-paramagnetic microparti-

cles that provide high binding capacity and fast magnetic response. Immobilized Metal

Affinity Chromatography (IMAC) purification employing NEBExpress Ni-NTA magnetic

beads can be performed under native or denaturing conditions, thereby allowing efficient

binding and purification of insoluble proteins, proteins that aggregate in inclusion bod-

ies, or proteins with tertiary structures that occlude the polyhistidine affinity tag. Low

non-specific binding properties permit immobilized fusion proteins to be used in reverse

purification schemes or in subsequent interaction experiments to capture or pull-down

protein complexes from crude cell lysates. Additionally, these beads enable screening of

expression and purification conditions to streamline functional and structural characteri-

zation of target proteins.

Binding Capacity:

Varies with target, typically ≥ 7.5 mg His-tagged fusion protein/ml bed volume

Ordering Information:

NEBExpress Ni-NTA Magnetic Beads (NEB #S1423S/L) ...........................1/5 ml

Polyhistidine-tagged Protein Puriﬁcation

NEBExpress Ni Resin

NEBExpress Ni Resin is an affinity matrix for the isolation and purification of polyhis-

tidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure

flow columns and batch purifications. NEBExpress Ni Resin is comprised of a highly

uniform and chemical-tolerant resin that is pre-charged with nickel ions on the matrix

surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, and com-

monly used reducing agents such as TCEP, DTT, and b-mercaptoethanol.

Binding Capacity:

1 ml of NEBExpress Ni Resin will bind ≥ 10 mg of His-tagged fusion protein

Ordering Information:

NEBExpress Ni Resin (NEB #S1428S)....................................................25 ml

features

• Intended for use in gravity or pressure flow columns and

batch purifications

• High specific binding yields purities of > 95% in a

single-purification step

• Strong nickel ion binding provides excellent resistance

to EDTA and reducing agents. Compatible with

commercially available detergent-based cell lysis

reagents.

• Can be used for isolation and purification of His-tagged

fusion proteins under native or denaturing conditions

HIS-TAG PURIFICATION

Companion Product:

TEV Protease

A highly-specific cysteine protease that is ideal for removal of affinity tags, such as

maltose binding protein (MBP) or poly-histidine (His-tag) from fusion proteins.

Ordering Information:

TEV Protease (NEB #P8112S) ......................................................1,000 units

NEBExpress Ni Spin Columns

NEBExpress Ni Spin columns are pre-packed with agarose-based microparticles rang-

ing in size from 10–100 µm for the small-scale isolation and purification of polyhisti-

dine-tagged (His-tagged) fusion proteins. Immobilized Metal Affinity Chromatography

(IMAC) purification employing NEBExpress Ni Spin columns can be performed under

native or denaturing conditions, including conditions in which EDTA or reducing

reagents are required, yielding highly pure target protein in a single purification step.

This enables screening of expression conditions and streamlines the functional and struc-

tural characterization of the target protein.

Binding Capacity:

Varies with target, ≥ 1 mg His-tagged fusion protein per column

Ordering Information:

NEBExpress Ni Spin Columns (NEB #S1427S/L)..........................10/25 columns

features

• Includes ready-to-use pre-packed Nispincolumns and all

required buffers forpurification

• Purify ≥ 1 mg His-tagged protein per column in as little as

15 minutes

• High specific binding yields purities of > 95% in a single

purification step

• Strong nickel ion binding provides excellent resistance

to EDTA, NaOH and reducing agents such as DTT and

b-mercaptoethanol. Compatible with commercially available

detergent-based cell lysis reagents.

NEBExpress Ni Spin Column Quick Start Protocol

1. Remove the bottom tab of the column and

place the column in a collection tube. Loosen the cap.

2. Centrifuge column at 800 x g for 1 minute.

Discard the buffer.

REMOVE STORAGE SOLUTION

3. Add 250 µl of Lysis/Binding Buffer.

4. Centrifuge column at 800 x g for 1 minute.

Discard the Lysis/Binding Buffer.

EQUILIBRATE COLUMN

5. Add up to 500 µl of sample lysate.

Tap to mix and allow to bind for 2 minutes.

6. Centrifuge column at 800 x g for 1 minute.

Retain flow through.

BIND LYSATE

7. Place the column in a new 2 ml centrifuge tube.

8. Add 250 µl of Wash Buffer to the column and

centrifuge at 800 x g for 1 minute.

Repeat twice.

WASH

9. Add 200 µl of Elution Buffer to the column.

Tap the column to mix.

10. Centrifuge at 800 x g for 1 minute. Retain the eluate.

Repeat once.

ELUTION

1. Remove the bottom tab of the column and

place the column in a collection tube. Loosen the cap.

2. Centrifuge column at 800 x g for 1 minute.

Discard the buffer.

REMOVE STORAGE SOLUTION

3. Add 250 µl of Lysis/Binding Buffer.

4. Centrifuge column at 800 x g for 1 minute.

Discard the Lysis/Binding Buffer.

EQUILIBRATE COLUMN

5. Add up to 500 µl of sample lysate.

Tap to mix and allow to bind for 2 minutes.

6. Centrifuge column at 800 x g for 1 minute.

Retain flow through.

BIND LYSATE

7. Place the column in a new 2 ml centrifuge tube.

8. Add 250 µl of Wash Buffer to the column and

centrifuge at 800 x g for 1 minute.

Repeat twice.

WASH

9. Add 200 µl of Elution Buffer to the column.

Tap the column to mix.

10. Centrifuge at 800 x g for 1 minute. Retain the eluate.

Repeat once.

ELUTION

HIS-TAG PURIFICATION

Maltose Binding Protein (MBP) Purification

Amylose Resin

Amylose resin is an affinity matrix used for the isolation of proteins fused to maltose-

binding protein (MBP). It is intended for use in a gravity flow column.

Binding Capacity:

> 4 mg MBP5*-paramyosin ΔSal fusion protein/ml amylose resin

Ordering Information:

Amylose Resin (NEB #E8021S/L)..................................................15/100 ml

features

• Highly specific binding of protein fused to MBP allows for

one-step purification

• Easily construct MBP-fusion proteins using the

NEBExpress MBP Fusion & Purification System

(NEB#E8201)

• MBP is easily removed by Factor Xa Protease

(NEB#P8010) or TEV Protease (NEB #P8112)

• Ideal for use in gravity flow column

• Can be regenerated and used multiple times

Amylose Resin High Flow

Amylose Resin High Flow is a cross-linked affinity matrix used for the isolation of

proteins fused to maltose-binding protein (MBP). This cross-linked, rigid matrix can be

used in automated chromatography systems.

Binding Capacity:

> 4 mg MBP5*-paramyosin ΔSal fusion protein/ml amylose resin high flow

Ordering Information:

Amylose Resin High Flow (NEB #E8022S/L) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15/100 ml

features

• Highly specific binding of protein fused to MBP allows for

one-step purification

• Easily construct MBP-fusion proteins using the

NEBExpress MBP Fusion & Purification System

(NEB#E8201)

• MBP is easily removed by Factor Xa Protease

(NEB#P8010) or TEV Protease (NEB #P8112)

• Ideal for use in automated chromatography systems

• Can be regenerated and used multiple times

Amylose Magnetic Beads

An affinity matrix for the small-scale isolation and purification of maltose-binding

protein (MBP) fusion proteins. Amylose is covalently coupled to a superparamagnetic

particle through a linkage that is stable and cleavage resistant over a wide pH range.

Binding Capacity:

10 µg MBP5*-paramyosin ΔSal fusion protein/mg amylose magnetic beads

Ordering Information:

Amylose Magnetic Beads (NEB #E8035S)...............................................25 mg

features

• Easily construct MBP-fusion proteins using the NEBExpress

MBP Fusion & Purification System (NEB #E8201)

• Quick, small-scale purification of MBP-fusion proteins

affords higher efficiency and enables high-throughput

workflows

• Immobilized fusion proteins can be used in subsequent

experiments to capture (pull down) target proteins from

crude cell lysates

MBP PURIFICATION

Anti-MBP Magnetic Beads

An affinity matrix for the small-scale isolation and purification of maltose-binding

protein (MBP) fusion proteins. Monoclonal anti-MBP is covalently coupled to 1 µm

nonporous superparamagnetic particles through a linkage that is stable and cleavage

resistant over a wide pH range, thereby permitting the immunomagnetic isolation of

MBP-fusion proteins from cell culture extracts.

Binding Capacity:

5 μg MBP5*-paramyosin ΔSal fusion protein/mg Anti-MBP magnetic beads

Ordering Information:

Anti-MBP Magnetic Beads (NEB #E8037S) ..............................................1 ml

features

• Immobilized fusion proteins can be used in subsequent

experiments to capture (pull down) target proteins that

specifically interact with the immobilized MBP-fusion protein

Anti-MBP Monoclonal Antibody

Anti-MBP Monoclonal Antibody is a murine anti-maltose binding protein (MBP)

antibody, isotype IgG2a. This antibody enables highly sensitive detection of nanogram

levels of MBP-fused proteins when used with a fluorophore-labeled Anti-Mouse IgG

secondary antibody.

Recommended Dilution:

1:10,000

Ordering Information:

Anti-MBP Monoclonal Antibody (NEB #E8032S/L).........................0.05/0.25 ml

features

• High purity and specificity for MBP-tag

• Verified for use in both Western blotting andELISA

MBP PURIFICATION

Chitin Magnetic Beads

An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding

domain (CBD). Chitin beads have been prepared with encapsulated magnetite, thereby

permitting the magnetic isolation of CBD-fusion proteins from cell culture supernatants.

Binding Capacity:

2 mg of CBD-fusion protein/ml bed volume

Ordering Information:

Chitin Magnetic Beads (NEB #E8036S/L)........................................... 5/25 ml

features

• CBD-fusion proteins can be easily constructed using the

IMPACT Purification System (NEB #E6901)

• Immobilized fusion proteins can be used in subsequent

experiments to capture (pull down) target proteins from crude

cell lysates

• Quick, small-scale purification of CBD-fusion proteins

enables higher throughput and efficiency

• Removal of CBD-tag during elution typically yields highly

pure, native protein

• The matrix can be regenerated

Anti-CBD Monoclonal Antibody

Anti-CBD Monoclonal Antibody is a murine anti-chitin binding domain (CBD) antibody,

isotype IgG1.

Recommended Dilution:

1:1,000

Ordering Information:

Anti-CBD Monoclonal Antibody (NEB #E8034S) ...................................0.05 ml

features

• High purity and specificity for chitin binding domain tag

• Verified for use in both Western blotting andELISA

features

• CBD-fusion proteins can be easily constructed using the

IMPACT Purification System (NEB #E6901)

• Strong specific binding for CBD-fusion protein enables

purification of highly pure protein from crude lysate in one step

• Removal of CBD-tag during elution typically yields highly pure,

native protein without the use of a protease

• This resin, when used with the pTXB1 Vector (NEB #N6707),

allows for the isolation of native recombinant proteins

possessing a reactive C-terminal thioester that can be used for

applications in intein-mediated protein ligation (IPL) and site-

specific labeling

• Resin may be regenerated up to 5 times

Chitin Resin

An affinity matrix for the isolation of target proteins fused to an intein-chitin binding

domain (CBD).

Binding Capacity:

2 mg maltose-binding protein (MBP)/mL bed volume released from the resin after

cleavage of the MBP-CBD-fusion

Ordering Information:

Chitin Resin (NEB #S6651S/L) ....................................................20/100 ml

Chitin Binding Domain (CBD) Purification

CBD PURIFICATION

features

• Extremely low non-specific binding of proteins from lysates

• Ideal for pull-down applications

• SNAP-tag fusion proteins can be labeled with SNAP-tag

substrates, including fluorophores and biotin

SNAP-Capture Pull Down Resin

An affinity matrix for the isolation of target proteins fused to a SNAP-tag. SNAP-Capture

Pull Down Resin is an agarose-based resin used to selectively capture and immobilize a

SNAP-tag fusion protein from solution. This resin consists of benzylguanine, a SNAP-tag

binding substrate, covalently attached to highly cross-linked agarose (4%).

Binding Capacity:

1 mg of SNAP-tag fusion protein/ml bed volume

Ordering Information:

SNAP-Capture Pull Down Resin (NEB #S9144S) .......................................2 ml

SNAP-Tag

Purification

features

• Extremely low non-specific binding of proteins from lysates

• Quick, small-scale purification of SNAP-tag fusion proteins

enables higher throughput and efficiency

• Ideal for pull-down applications

• SNAP-tag fusion proteins can be labeled with SNAP-tag

substrates, including fluorophores and biotin

SNAP-Capture Magnetic Beads

An affinity matrix for the small-scale isolation of target proteins fused to a SNAP-tag.

SNAP-Capture Magnetic Beads are used to selectively immobilize and magnetically

separate a SNAP-tag fusion protein from solution using magnetic agarose beads. They

are prepared by the coupling of SNAP-tag substrate benzylguanine with highly stable

20-100 µm superparamagnetic particles.

Binding Capacity:

≥ 1 mg of target SNAP-tag fusion protein per ml of bead bed volume

Ordering Information:

SNAP-Capture Magnetic Beads (NEB #S9145S).........................................2 ml

features

• Simplify transcriptomics workflows by eliminating total

RNA isolation steps and simply isolating mRNA from crude

samples

• The magnetic separation technology is scalable and

permits elution of intact mRNA in small volumes, thereby

eliminating the need to precipitate isolated mRNA

• Covalent linkage of oligo d(T)

to the beads allows them

to be reused, thereby enabling multiple isolations from the

same sample input

• Elution of isolated mRNA is optional; the bound d(T)

can

be used as a primer for reverse transcriptase in first-strand

cDNA reactions

Oligo d(T)

Magnetic Beads

Oligo d(T)

Magnetic Beads consist of oligo d(T)

covalently coupled to 1 μm

superparamagnetic particle through a linkage that is stable over a wide pH range. These

beads enable small-scale isolations of mRNA from a variety of samples, including in vitro

transcribed mRNA, total RNA, crude cell lysates and tissue. The selectivity for mRNA

results from the annealing of bead-linked oligo d(T)

to the poly(A) region present in

most eukaryotic mRNAs.

Binding Capacity:

≥ 5 µg rA

per mg of beads

Ordering Information:

Oligo d(T)

Magnetic Beads (NEB #S1419S)............................................5 ml

Magnetic Bead Purification Products

SNAP-TAG PURIFICATION

Streptavidin Magnetic Beads

Streptavidin Magnetic Beads are 1 µm superparamagnetic particles covalently coupled

toa highly pure form of streptavidin. The beads provide fast magnetic response times

and reaction kinetics, and they have high binding capacity and sensitivity while

retainingtheir physical integrity. They can be used to capture biotin-labeled substrates

including DNA, RNA, peptides, antigens, antibodies and other proteins of interest

in manual or automated workflows. These beads typically exhibit lower non-specific

binding of proteins.

Binding Capacity:

≥ 30 µg biotinylated antibody per mg of beads or > 500 pmol of single-stranded 25 bp

biotinylated oligonucleotide per mg of beads

Ordering Information:

Streptavidin Magnetic Beads (NEB #S1420S).............................................5 ml

features

• Strong biotin-streptavidin interaction

(Ka= 10

-1

), coupled with low, non-specific binding

of streptavidin, permits captured substrates to be

useful as ligands in sample preparation, nucleic acid

isolation,immunoprecipitations and proteomics workflows

• Can be used for solution-phase panning in phage display

experiments, SELEX, purification of DNA/RNA binding

proteins and cell-based screening

• Provided in an RNase-free solution

features

• Can be used for manual processing of multiple samples or

automated for high-throughput applications

• Magnetic separation technology enables elution of

intact mRNA in small volumes, eliminating the need for

precipitating the poly(A)+ transcripts in the eluent

• Intact poly(A)+ RNA isolated in less than one hour

• Oligo d(T)

Magnetic Beads can be reused up to three times

with the same sample input

Magnetic mRNA Isolation Kit

The Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells

and tissue without requiring phenol or other organic solvents. The technology is based

on the coupling of Oligo d(T)

to 1 μm paramagnetic beads, which is then used as the

solid support for the direct binding of poly(A)+ RNA.

Ordering Information:

Magnetic mRNA Isolation Kit (NEB #S1550S) ................................ 25 isolations

Hydrophilic Streptavidin Magnetic Beads

Hydrophilic Streptavidin Magnetic Beads are 2–3 µm superparamagnetic particles cova-

lently coupled to a highly pure form of streptavidin. The beads provide rapid magnetic

response times and reaction kinetics, and they have high binding capacity and sensitivity

while retaining their physical integrity. They can be used to capture biotin-labeled sub-

strates including DNA, RNA, peptides, antigens, antibodies and other proteins of interest

in manual or automated workflows. These beads typically exhibit lower non-specific

binding of nucleic acids.

Binding Capacity:

> 400 pmol of single-stranded 25 bp biotinylated oligonucleotide per mg of beads

Ordering Information:

Hydrophilic Streptavidin Magnetic Beads (NEB #S1421S) ............................. 5 ml

features

• Strong biotin-streptavidin interaction

(Ka=10

-1

), coupled with low, non-specific binding

of streptavidin, permits captured substrates to be

useful as ligands in sample preparation, nucleic acid

isolation,immunoprecipitations and proteomics workflows

• Can be used for solution-phase panning in phage display

experiments, SELEX, purification of DNA/RNA binding

proteins and cell-based screening

• Provided in an RNase-free solution

MAGNETIC BEAD PURIFICATION

Protein A and Protein G Magnetic Beads

Protein A Magnetic Beads are 2–3 µm superparamagnetic particles covalently cou-

pled to a highly pure form of recombinant protein A. The beads allow for isolation of

most mammalian immunoglobulins (IgGs) and are amenable to immunoprecipitation.

Predominant Fc-binding allows optimal IgG orientation upon binding to the outer sur-

face of the Protein A Magnetic Beads allowing Fab regions to efficiently bind antigen.

Protein G Magnetic Beads are 2–3 µm superparamagnetic particles covalently coupled

to a highly pureform of recombinant protein G. The beads allow for isolation of

most mammalian immunoglobulins(IgGs) and are amenable to immunoprecipitation.

Predominant Fc-binding allows optimal IgG orientation upon binding to the outer

surface of the Protein G Magnetic Beads allowing Fab regions to efficiently bind antigen.

These beads can be used to immunoprecipitate target proteins from crude cell lysates

using a selected primary antibody. In addition, specific antibodies can be chemically

cross-linked to the Protein A- or Protein G- coated surface to create a reusable

immunoprecipitation bead, thereby avoiding the co-elution of antibody with the target

antigen.

Binding Capacity:

> 280 µg of Human IgG per ml of beads

Ordering Information:

Protein A Magnetic Beads (NEB #S1425S) ...............................................1 ml

Protein G Magnetic Beads (NEB #S1430S)

...............................................1 ml

features

• Exhibits high affinity for subclasses of IgG from many

species, including human, rabbit, mouse, rat and sheep

• Protein G can be used for immuno-precipitations with

mouse monoclonal antibodies

• Protein coupling is stable and cleavage resistant over a wide

pH range. This permits the immunomagnetic purification of

IgGs from ascites, serum or cell culture supernatants

• Can be regenerated without loss of bindingcapacity

• Quick, small-scale purification of many subclasses of IgG

affords higher throughput and efficiency

Goat Anti-Mouse IgG Magnetic Beads

An affinity matrix for the small-scale immunomagnetic separation and purification of

mouse IgG. Specifically, the beads consist of Anti-Mouse IgG that is covalently coupled

to a 1 μm nonporous superparamagnetic particle.

Binding Capacity:

5 μg mouse lgG/mg Goat Anti-Mouse IgG Beads

Ordering Information:

Goat Anti-Mouse IgG Magnetic Beads (NEB #S1431S)...............................20 mg

features

• This secondary antibody binds the heavy chain of mouse

IgG and is suitable for immunoassays that employ a mouse

IgG primary monoclonal antibody

• Cell separations and sorting can be accomplished using a

mouse IgG antibody to defined cell surface antigens

• Quick, small-scale purification of mouse IgG affords higher

throughput and efficiency

Goat Anti-Rabbit IgG Magnetic Beads

An affinity matrix for the small-scale immunomagnetic separation and purification of

rabbit IgG. Specifically, the beads consist of Goat Anti-Rabbit IgG that is covalently

coupled to a 1 μm nonporous superparamagnetic particle.

Binding Capacity:

5 μg rabbit lgG/mg Goat Anti-Rabbit IgG Magnetic Beads

Ordering Information:

Goat Anti-Rabb IgG Magnetic Beads (NEB #S1432S) ..................................1 ml

features

• This secondary antibody binds the heavy chain of all rabbit

IgG subclasses and is suitable for immunoassays that

employ a rabbit IgG primary polyclonal antibody

• Cell separations and sorting can be accomplished using a

rabbit IgG antibody to defined cell surface antigens

• Quick, small-scale purification of rabbit IgG affords higher

throughput and efficiency

MAGNETIC BEAD PURIFICATION

Goat Anti-Rat IgG Magnetic Beads

An affinity matrix for the small-scale immunomagnetic separation and purification

of rat IgG. Specifically, the beads consist of anti-Rat IgG that is covalently coupled to a

1 μm nonporous superparamagnetic particle.

Binding Capacity:

5 μg rat IgG/mg of Goat Anti-Rat IgG Magnetic Beads

Ordering Information:

Goat Anti-Rat IgG Magnetic Beads (NEB #S1433S).....................................1 ml

features

• This secondary antibody binds the Fc portion of

all monoclonal rat IgG subclasses and is suitable

for immunoassays that employ a rat IgG primary

monoclonalantibody

• Cell separations and sorting can be accomplished using

a rat IgG antibody to defined cell surface antigens

• Quick, small-scale purification of rat IgG affords higher

throughput and efficiency

Magnetic Separation Racks

APPLICATION MAGNETS CAPACITY CONVENIENCE

6-Tube Magnetic

Separation Rack

(NEB #S1506)

Designed for small-scale separations using

magnetic particles

Neodymium rare earth permanent

magnets

6 tubes

(1.5 ml)

Use with magnetic particle-based affinity purification for

rapid, small-scale purifications

50 ml Magnetic

Separation Rack

(NEB #S1507)

Designed for small-scale separations using

magnetic particles

Neodymium rare earth permanent

magnets

4 tubes

(50 ml)

Use with magnetic particle-based affinity purification for

rapid, streamlined purifications

12-Tube Magnetic

Separation Rack

(NEB #S1509)

Designed for small-scale separations using

magnetic particles

Neodymium rare earth permanent

magnets

12 tubes

(1.5 ml)

Use with magnetic particle-based affinity purification for

rapid, small-scale purifications

96-Well Microtiter

Plate Magnetic

Separation Rack

(NEB #S1511)

Designed for use with commercially

available high-flanged 100 µl to 300µl

flat-bottom 96-well microplates

24 side-pull magnetic pins attract

magnetic beads from solution to the

side walls of four adjacent wells

96-well

The orientation of the magnetic field ensures complete

removal of the magnetic beads from solution during

pipetting steps, thereby minimizing sample loss

NEBNext

Magnetic

Separation Rack

(NEB #S1515)

Designed for rapid and effective small-

scale separations of magneticparticles

Anodized aluminum rack with

Neodymium Iron Boron (NdFeB) rare

earth magnets

24 tubes

(0.2 ml)

Next generation sequencing library preparation workflows

include magnetic bead-based purification and size-selection

steps. It is important for library yield and quality that bead

separation be highly efficient and fast, and this is enabled

by the powerful fixed magnet cores in this rack.

MAGNETIC BEAD PURIFICATION

RES_BEADS – Version 1.0 – 11/19

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New England Biolabs (UK) Ltd

75-77 Knowl Piece

Wilbury Way

Hitchin

Herts

SG4 0TY

Free Phone (General & Orders): 0800 318486

Free Phone (Technical Support): 0800 6522890

Free Phone (Accounts): 0800 6522891

Free Fax: 0800 435682

Email (General): info.uk@neb.com

Email (Orders): orders.uk@neb.com

Email (Technical Support): techsupport.u[email protected]

Email (Accounts): accounts.uk@neb.com

USA

New England Biolabs, Inc.

Telephone (978) 927-5054

Toll Free (USA Orders) 1-800-632-5227

Toll Free (USA Tech) 1-800-632-7799

Fax (978) 921-1350

e-mail: info@neb.com

www.neb.com

For UK prices visit:

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