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INTRODUCTION
The AlamarBlue® Cell Viability Assay Reagent is used to quantify cellular metabolic
activity and in turn determine the concentration of viable cells in a given sample. The
supplied reagent can be used to measure the cell growth kinetics i.e. the growth of a cell
population over time by measuring it at two or more time points of the sample(s).
AlamarBlue Cell Viability Reagent quantitatively measures the proliferation of
mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction
(REDOX) indicator that both fluoresces and change color in response to the chemical
reduction due to cell growth. Since, the AlamarBlue is very stable and nontoxic to the
cells, continuous monitoring of cultures over time is possible.
The alamarBlue dye in its oxidized form is blue in color and non-fluorescent. In
alamarBlue Reagent assay, the growing cells cause a chemical reduction of the
alamarBlue dye from non-fluorescent blue to fluorescent red. The continued growth of
viable cells maintain a reducing environment (fluorescent, red) and inhibition of growth
maintains an oxidized environment (non-fluorescent, blue), which can be detected using
a fluorescence or absorbance detector.
Conversion of alamarBlue dye from oxidized to reduced form
ITEM(S) SUPPLIED
Cat. # Description Size
786-921 AlamarBlue Cell Viability Reagent 10ml
786-922 AlamarBlue Cell Viability Reagent 25ml
786-923 AlamarBlue Cell Viability Reagent 50ml
STORAGE CONDITION
It is shipped at ambient temp. Upon arrival, store it at 4°C, protected from light. The
reagent is stable for six months, when stored and used as recommended.